Journal: Vaccines

Article Title: Analytical Performance of a Multiplexed Microarray Assay for Rapid Identification and Quantification of a Multivalent mRNA Vaccine

doi: 10.3390/vaccines12101144

Figure Lengend Snippet: ( a ) Schematic of each mRNA construct with naming and binding locations of the capture oligos. Performance results for blue-shaded capture oligos are the focus of this work. ( b ) Microarray layout where each capture oligo was printed in triplicate in three locations on the microarray (n = 9 spots). ( c ) Schematic of the assay detection principle, in which each printed capture oligo can bind an mRNA construct in a sequence-specific manner and be fluorescently labeled via the polyA tail. ( d ) Representative fluorescence images of each monovalent encapsulated mRNA construct captured and labeled on the microarray, as well as a quadrivalent mixture of the four mRNA constructs, each at a sample concentration of 20 µg/mL. The relevant capture oligos are highlighted for each monovalent sample, with brighter green indicating a higher fluorescence signal.

Article Snippet: Capture oligos were printed onto epoxide-functionalized glass slides at InDevR using a piezoelectric microarray printing system.

Techniques: Construct, Binding Assay, Microarray, Sequencing, Labeling, Fluorescence, Concentration Assay